Recent Publications from the Isberg Lab

Research Publications Genetics Microbiology


de Jesús-Díaz DA, Murphy C, Sol A, Dorer M, Isberg RR. 2017. Host cell S phase restricts Legionella pneumophila intracellular replication by destabilizing the membrane-bound replication compartment. MBio 8: e02345-16.. books

Mitchell G, Isberg RR 2017. Innate immunity to intracellular pathogens: Balancing microbial elimination and inflammation. Cell Host Microboe 22: 166-175. books

Geisinger E, Isberg RR. 2017. Interplay between antibiotic resistance and virulence during disease promoted by multidrug-resistant bacteria. J Infect. Dis. 215(suppl_1): S9-S17. books

Kotewicz KM, Ramabhadran V, Sjoblom N, Vogel JP, Haensslbookser E, Zhang M, Behringer J, Scheck RA, Isberg RR. 2016. A single Legionella effector catalyzes a multistep ubiquitination pathway to rearrange tubular endoplasmic reticulum for replication. Cell Host Microbe Epub ahead of print.

Sheehan KB, Martin M, Lesser CF, Isberg RR, Newton IL. 2016. Identification and characterization of a candidate Wolbachia pipientis type IV effector that interacts with the actin cytoskeleton. MBio 7: e00622-e00216. books

Shen H, Gonzalez-Juarbe N, Blanchette K, Crimmins G, Bergman MA, Isberg RR, Orihuela CJ, Dube PH. 2016. CD8+ T cells specific to a single Yersinia pseudotuberculosis epitope restrict bacterial replication in the liver but fail to provide sterilizing immunity. Infect Genet Evol. Epub ahead of print. books

Davis KM, Isberg RR. 2016. Defining heterogeneity within bacterial populations via single cell approaches. Bioessays Epub ahead of print. books

O'Connor TJ, Zheng H, VanRheenen SM, Ghosh S, Cianciotto NP, Isberg RR. 2016. Iron limitation triggers early egress by the intracellular bacterial pathogen Legionella pneumophila. Infect Immun. Epub ahead of print.  books

Hempstead AD, Isberg RR 2015. Inhibition of host cell translation elongation by Legionella pneumophila blocks the host cell unfolded protein response. Proc Natl Acad Sci USA 112: E6790-E6797.   books

Chen Y, Lin Y, Davis KM, Wang Q, Rnjak-Kovacina J, Li C, Isberg RR, Kumamoto CA, Mecsas J, Kaplan DL 2015. Robust bioengineered 3D functional human intestinal epithelium. Sci Rep. 5: 13708.   books

Isaac DT, Laguna RK, Valtz N, Isberg RR. 2015. MavN is a Legionella pneumophila vacuole-associated protein required for efficient iron acquisition during intracellular growth. Proc Natl Acad Sic USA 112: E5208-E5217.  books

Haenssler E, Ramabhadran V, Murphy CS, Heidtman MI, Isberg RR. 2015. Endoplasmic reticulum tubule protein Reticulon 4 associates with the L. pneumophila vacuole and with translocated substrate Ceg9. Infect Immun. 83: 3479-3489.  books

Asrat S, Davis KM, Isberg RR. 2015. Modulation of the host innate immune and inflammatory response by translocated bacterial proteins. Cell Microbiol. 17: 785-795. books

Sheahan KL, Isberg RR. 2015.Identification of mammalian proteins that collaborate with type III secretion system function: Involvement of a chemokine receptor in supporting translocon activity. MBio 6: e02023-14. books

Geisinger E, Isberg RR 2015. Antibiotic modulation of capsular exopolysaccharide and virulence in Acinetobacter baumannii. PLoS Pathog 11: e1004691. books

Davis KM, Mohammadi S, Isberg RR. 2014. Community behavior and spatial regulation within a bacterial microcolony in deep tissue sites serves to protect against host attack. Cell Host Microbe 17: 21-31. books

Davis KM, Isberg RR 2014. Plague's partners in crime. Immunity 41: 347-349. books

Asrat S, de Jesús DA, Hempstead AD, Ramabhadran V, Isberg RR. 2014. Bacterial pathogen manipulation of host membrane trafficking. Ann Rev Cell Dev Biol. 30: 79-109. books

Asrat S, Dugan AS, Isberg RR 2014. The frustrated host response to Legionella pneumophila Is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PLoS Pathog. 10: e1004229. books

O'Connor TJ, Isberg RR. 2014. iMAD, a genetic screening strategy for dissecting complex interactions between a pathogen and its host. Nat Protoc. 9: 1916-1930. books

Isaac DT, Isberg R. 2014. Master manipulators: an update on Legionella pneumophila Icm/Dot translocated substrates and their host targets. Front Microbiol. 9: 343-359. books

Creasey EA, Isberg RR. 2014. Maintenance of vacuole integrity by bacterial pathogens. Curr Opin Microbiol. 17C: 46-52. books

Hempstead AD, Isberg RR. 2013. Host signal transduction and protein kinases Implicated in Legionella infection. Curr Top Microbiol Immunol. 376: 249-269. books

Amyot WM, Dejesus D, Isberg RR. 2013. Poison domains block transit of translocated substrates via the Legionella pneumophila Icm/Dot system. Infect Immun. 81: 3239-3252. books  Correction: Infect Immun. 82: 4436.

Farelli JD, Gumbart JC, Akey IV, Hempstead A, Amyot W, Head JF, McKnight CJ, Isberg RR, Akey CW.2013. IcmQ in the type 4b secretion system contains an NAD(+) binding domain. Structure 21: 1361-1373. books

De Jesús DA, O'Connor TJ, Isberg RR. 2013. Analysis of Legionella infection using RNAi in Drosophila cells. Methods Mol Biol. 954: 251-264. books

Mohammadi S, Isberg RR. 2013. Cdc42 interacts with the exocyst complex to promote phagocytosis. J Cell Biol. 200: 81-93. books

O'Connor TJ, Boyd D, Dorer MS, Isberg RR. 2012. Aggravating genetic interactions allow a solution to redundancy in a bacterial pathogen. Science 338: 1440-1444. books

De Jesús DA, O'Connor TJ, Isberg RR. 2012. Analysis of Legionella Infection Using RNAi in Drosophila Cells. Methods Mol Biol. 954: 251-264. books

Choy A, Dancourt J, Mugo B, O'Connor TJ, Isberg RR, Melia TJ, Roy CR. 2012. The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation. Science 338: 1072-1076 books

Liu M, Haenssler E, Uehara T, Losick VP, Park JT, Isberg RR. 2012. The Legionella pneumophila EnhC protein interferes with immunostimulatory muramyl peptide production to evade innate immunity. Cell Host Microbe 12: 166-176. books

Crimmins GT, Mohammadi S, Green ER, Bergman MA, Isberg RR, Mecsas J. 2012. Identification of MrtAB, an ABC transporter specifically required for Yersinia pseudotuberculosis to colonize the mesenteric lymph nodes. PLoS Pathog. 8: e1002828. books

Ensminger AW, Yassin Y, Miron A, Isberg RR. 2012. Experimental evolution of Legionella pneumophila in mouse macrophages leads to strains with altered determinants of environmental survival. PLoS Pathog. 8: e1002731 books

Sarantis H, Balkin DM, De Camilli P, Isberg RR, Brumell JH, Grinstein S. 2012. Yersinia entry into host cells requires Rab5-dependent dephosphorylation of PI(4,5)P₂ and membrane scission. Cell Host Microbe 11: 117-128. books

Creasey EA, Isberg RR.  2012. The protein SdhA maintains the integrity of the Legionella-containing vacuole. Proc Natl Acad Sci USA 109: 3481-3486. books

Sarantis H, Balkin DM, De Camilli P, Isberg RR, Brumell JH, Grinstein S.  2012. Yersinia entry into host cells requires Rab5-dependent dephosphorylation of PI(4,5)P(2) and membrane scission. Cell Host Microbe. 11: 117-128. books

Crimmins GT, Isberg RR. 2012. Analyzing microbial disease at high resolution: following the fate of the bacterium during infection. Curr Opin Microbiol. 15: 23-27. books

O'Connor TJ, Adepoju Y, Boyd D, Isberg RR. 2011. Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host range expansion. Proc Natl Acad Sci U S A. 108: 14733-14740. books

Huang L, Boyd D, Amyot WM, Hempstead AD, Luo ZQ, O'Connor TJ, Chen C, Machner M, Montminy T, Isberg RR. 2011. The E Block motif is associated with Legionella pneumophila translocated substrates. Cell Microbiol. 13: 227-245. books

Ensminger AW, Isberg RR. 2010. E3 ubiquitin ligase activity and targeting of BAT3 by multiple Legionella pneumophila translocated substrates. Infect Immun. 78: 3905-3919. books

Rajagopal S, Ji Y, Xu K, Li Y, Wicks K, Liu J, Herman IM, Wong K-W, Isberg RR, and Buchsbaum RJ. 2010. Scaffold proteins IRSp53 and spinophilin regulate localized Rac activation by Tiam1. J Biol Chem. 285: 18060-180671. books

Losick VP, Haenssler E, Moy MY, Isberg RR. 2010. LnaB: a Legionella pneumophila activator of NF-κB. Cell Microbiol. 12: 1083-1097. books

Auerbuch V, Golenbock DT, Isberg RR. 2009. Innate immune recognition of Yersinia pseudotuberculosis type III secretion. PLoS Pathog. 5: e1000868. books

Li Z, Dugan AS, Bloomfield G, Skelton J, Ivens A, Losick V, Isberg RR. 2009. The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA. Cell Host Microbe 17: 253-267.  books

Bergman MA, Loomis WP, Mecsas J, Starnbach MN, Isberg RR. 2009. CD8(+) T cells restrict Yersinia pseudotuberculosis infection: bypass of anti-phagocytosis by targeting antigen-presenting cells. PLoS Pathog. 5: 1000573. books

Mohammadi S, Isberg RR. 2009. Yersinia pseudotuberculosis virulence determinants invasin, YopE, and YopT modulate RhoG activity and localization. Infect Immun. 77: 4771-4782. books

Vance RE, Isberg RR, Portnoy DA. 2009. Patterns of pathogenesis: discrimination of pathogenic and nonpathogenic microbes by the innate immune system. Cell Host Microbe 6: 10-21. books

Ensminger AW, Isberg RR. 2009. Legionella pneumophila Dot/Icm translocated substrates: a sum of parts. Curr Opin Microbiol. 12: 67-73. books

Heidtman M, Chen EJ, Moy MY, Isberg RR. 2009. Large-scale identification of Legionella pneumophila Dot/Icm substrates that modulate host cell vesicle trafficking pathways. Cell Microbiol. 11: 230-248. books

Isberg RR, O'Connor TJ, Heidtman M. 2009. The Legionella pneumophila replication vacuole: making a cosy niche inside host cells. Nat Rev Microbiol. 7: 13-24. books

Raychaudhury S, Farelli JD, Montminy TP, Matthews M, Ménétret JF, Duménil G, Roy CR, Head JF, Isberg RR, Akey CW. 2009. Structure and function of interacting IcmR-IcmQ domains from a type IVb secretion system in Legionella pneumophila. Structure 15: 590-601. books

Losick VP, Stephan K, Smirnova II, Isberg RR, Poltorak A. 2009. A hemidominant Naip5 allele in mouse strain MOLF/Ei-derived macrophages restricts Legionella pneumophila intracellular growth. Infect Immun. 77: 196-204. books

Wong KW, Mohammadi S, Isberg RR. 2008. The polybasic region of Rac1 modulates bacterial uptake independently of self-association and membrane targeting. J Biol Chem. 283: 35954-35965. books

Liu M, Conover GM, Isberg RR. 2008. Legionella pneumophila EnhC is required for efficient replication in tumour necrosis factor alpha-stimulated macrophages. Infect Immun. 76: 1906-1923. books

Shin OS, Isberg RR, Akira S, Uematsu S, Behera AK, Hu LT. 2008. Distinct roles for MyD88 and Toll-like receptors 2, 5, and 9 in phagocytosis of Borrelia burgdorferi and cytokine induction. Infect Immun. 76: 2341-2351. books

Conover GM, Martinez-Morales F, Heidtman MI, Luo ZQ, Tang M, Chen C, Geiger O, Isberg RR. 2008. Phosphatidylcholine synthesis is required for optimal function of Legionella pneumophila virulence determinants. Cell Microbiol. 10: 514-512. books

Machner MP, Isberg RR. 2007. A bifunctional bacterial protein links GDI displacement to Rab1 activation. Science 318: 974-977.  books

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